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Pixel perfect: a real-time image processing system for biology.(IMAGING)

By Budge, Scott; Hopkins, Derek; LaMarche, Brian; Perrine, Kenneth; Sowa, Marianne
Publication: Scientific Computing & Instrumentation
Date: Thursday, September 1 2005

Scientific visioning systems often rely upon pixel-perfect precision to produce meaningful data. Cutting-edge equipment used in the study of cell signaling is no exception; proper image alignment is critical for successful experiments. Biologists at Pacific Northwest National Laboratory (PNNL) put together a special multispectral confocal microscope that was capable of acquiring live images of cells and proteins using two simultaneous spectral channels. But there was a problem: the dual images resembled poorly registered Sunday comics and were of limited use. This article describe

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